The Pharmacy Guild of Australia was established in 1928, bringing together several small retail pharmacy organisations then operating in the various States. The Guild is registered under the federal Workplace Relations Act 1996 as an employers' organisation. Its members are owners of some 4,500 pharmacies throughout Australia.

The Guild is a national employers' organisation which functions as a single legal entity rather than a federation. The development of policy is the responsibility of the Guild's supreme governing body, the National Council, on which all State and Territory Branches are represented. Implementation of policy is overseen by the National Executive and effected through the National Secretariat.